Diagnostic Bioluminescent Phage for Detection of Yersinia pestis
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چکیده
منابع مشابه
Diagnostic bioluminescent phage for the detection of Yersinia pestis
3 David A. Schofield*, Ian J. Molineux, and Caroline Westwater 4 5 6 Guild Associates, Inc., Charleston, South Carolina, 29407; Molecular Genetics and 7 Microbiology, Institute for Cellular and Molecular Biology, University of Texas at Austin, 8 Texas, 78712; 9 Department of Craniofacial Biology, Medical University of South Carolina, South Carolina, 1
متن کاملDiagnostic bioluminescent phage for detection of Yersinia pestis.
Yersinia pestis is the etiological agent of the plague. Because of the disease's inherent communicability, rapid clinical course, and high mortality, it is critical that an outbreak, whether it is natural or deliberate, be detected and diagnosed quickly. The objective of this research was to generate a recombinant luxAB ("light")-tagged reporter phage that can detect Y. pestis by rapidly and sp...
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BACKGROUND Most Yersinia pestis strains are known to express a capsule-like antigen, fraction 1 (F1)(.) F1 is encoded by the caf1 gene located on the large 100-kb pFra plasmid, which is found in Y. pestis but not in closely related species such as Yersinia enterocolytica and Yersinia pseudotuberculosis. In order to find antibodies specifically binding to Y. pestis we screened a large single cha...
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Yersinia pestis, the plague bacillus, has an exceptional pathogenicity for humans. The plague bacillus emerged very recently (≈3,000 years ago) from the enteropathogen Y. pseudotuberculosis. Early after its emergence, Y. pestis became infected by a filamentous phage named YpfΦ. During the microevolution of the plague bacillus, the phage remained in the various lineages as an unstable extrachrom...
متن کاملDevelopment of Bioluminescent Bioreporters for In Vitro and In Vivo Tracking of Yersinia pestis
Yersinia pestis causes an acute infection known as the plague. Conventional techniques to enumerate Y. pestis can be labor intensive and do not lend themselves to high throughput assays. In contrast, bioluminescent bioreporters produce light that can be detected using plate readers or optical imaging platforms to monitor bacterial populations as a function of luminescence. Here, we describe the...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2009
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.01533-09